Effects of glycated albumin on the expression of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells

Zhang Li, Shen Cheng-xing, Feng Yi, Ma Gen-shan

Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing   210009, Jiangsu Province, China

Zhang Li★, Master, Attending physician, Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing   210009, Jiangsu Province, China
lilynanjing@163.com

Correspondence to: Ma Gen-shan, Doctor, Tutor of doctor, Department of Cardiology, Zhongda Hospital, Southeast University, Nanjing   210009, Jiangsu Province, China
magenshan@seu.edu.cn

Supported by: Open Program of Jiangsu Provincial Health Bureau, No. WK0501*

Received: 2007-11-01
Accepted: 2008-03-03

Abstract
BACKGROUND: Previous study reveals that, glycated albumin plays an important role on the apoptosis of endothelial cells and the expression of monocyte chemoattractant protein-1 through increasing the activity of mitogen activated protein kinase and protein tyrosine kinase, besides generating active oxygen products.
OBJECTIVE: To investigate the effects of glycated albumin on the expression of monocyte chemoattractant protein-1 in the cultured human umbilical vein endothelial cells (HUVECs).
DESIGN, TIME AND SETTING: A single sample observation was carried out in the Cardiovascular Laboratory of Southeast University (Nanjing, Jiangsu, China) from May to November in 2006.
MATERIALS: ECV304 HUVEC strain was provided from Shanghai Institute of Cell Biology, Chinese Academy of Sciences (China).
METHODS: Experimental procedures were assigned to two parts. On one hand, HUVECs were cultured with glycated albumin of the concentration of 400 mg/L for 0, 8, 16, 24, 48, or 72 hours. On the other hand, HUVECs were cultured with glycated albumin of the concentrations of 100, 200, 400, and 800 mg/L for 24 hours. In the control group, HUVECs were incubated with bovine serum albumin of the concentration of 400 mg/L and RPMI 1640 culture medium without addition of the serum.
MAIN OUTCOME MEASURES: The proliferation of HUVECs was estimated by MTT colorimetric assay. The content of monocyte chemoattractant protein-1 in the supernatant was measured by enzyme-linked immunosorbent assay.
RESULTS: Glycated albumin inhibited the proliferation rate of HUVECs in a time-dependent manner (P < 0.05). The proliferation of cells cultured for 8 hours were not remarkably inhibited. However, the proliferation inhibiting effect on the cells cultured for 48 hours was significantly higher than that cultured for 72 hours. Compared to RPMI 1640 group and bovine serum albumin group, the proliferation inhibiting effect on cells cultured with 400 or 800 mg/L glycated albumin was significantly enhanced (P < 0.05). This increment suggested a dose-dependent manner. The expression of monocyte chemoattractant protein-1 in HUVECs cultured with 400 mg/L glycated albumin for 6 and 12 hours were significantly increased (P < 0.05) and reached a peak with 800 mg/L glycated albumin. Glycated albumin increased the expression of monocyte chemoattractant protein-1 in a time- and dose-dependent manner.
CONCLUSION: Glycated albumin inhibits the proliferation of cultured HUVECs in a time- and dose-dependent manner. To some extent, it also increases the expression of monocyte chemoattractant protein-1 in HUVECs at a time-dependent manner.

Zhang L, Shen CX, Feng Y, Ma GS.Effects of glycated albumin on the expression of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(28):5422-5426
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