Influence of tissue-engineered periosteum constructed by porcine small intestinal submucosa scaffold combined with rabbit mesenchymal stem cells on T lymphocyte proliferation

Liu Jia1, Xu Hong-wen2, Wang Shuan-ke2, Fu Wen1

1Seventh Department of Surgery, Guangzhou Children's Hospital, Guangzhou 510120, Guangdong Province, China; 2Department of Orthopaedics, Second Affiliated Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China

Liu Jia★, Master, Physician, Seventh Department of Surgery, Guangzhou Children's Hospital, Guangzhou 510120, Guangdong Province, China

Received: 2008-04-01
Accepted: 2008-06-30

Abstract
BACKGROUND: Small intestinal submucosa is an acellular collagen layer, which contains many growth factors and is similar to the periosteum, comprising type Ⅰ and Ⅲ fibrous collagen proteins.
OBJECTIVE: To investigate the influence of rabbit tissue-engineered periosteum constructed by porcine small intestinal submucosa (PSIS) scaffold on the proliferation of peripheral blood T lymphocytes, and to approach immunological property of rabbit tissue-engineered periosteum.
DESIGN, TIME AND SETTING: A controlled observation of in vitro cytology was performed in the Institute of Orthopaedics, the Second Affiliated Hospital of Lanzhou University (Lanzhou, Gansu, China) between September 2005 and March 2007.
MATERIALS: Healthy porcine small intestine and four New Zealand purebred rabbits of 20 days old were used in this study.
METHODS: By means of precipitation method, rabbit tissue-engineered periosteum was constructed using co-cultured osteoblasts derived from mesenchymal stem cells as seed cells and PSIS as scaffolds. Peripheral blood T lymphocytes were used as the effector cells in mixed lymphocyte reactions, and then cultured with three stimulus groups, namely osteoblastic induction group (induction of mesenchymal stem cells for 14 days), tissue-engineered periosteum group (induction of mesenchymal stem cells for 14 days combined with PSIS scaffold) and PSIS group (PSIS scaffold alone). Afterwards, they were added to the ongoing one-way mixed lymphocyte reactions.
MAIN OUTCOME MEASURES: The proliferation of T lymphocytes in peripheral blood was tested using MTT method in vitro.
RESULTS: The inhibition ratio of T lymphocytes in the osteoblastic induction, tissue-engineered periosteum and PSIS groups was (11±3)%, (23±5)% and (48±10)%, respectively. The T lymphocytes inhibition ratio was significantly different among three stimulus groups (P < 0.01). The osteoblastic induction group was not significantly different from the tissue-engineered periosteum group (P > 0.05).
CONCLUSION: Rabbit tissue-engineered periosteum can inhibit the proliferation of T lymphocytes in peripheral blood, and has immunoregulatory property as same as osteoblasts derived from mesenchymal stem cells.

Liu J, Xu HW, Wang SK, Fu W.Influence of tissue-engineered periosteum constructed by porcine small intestinal submucosa scaffold combined with rabbit mesenchymal stem cells on T lymphocyte proliferation.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(27):5239-5242 [www.zglckf.com/zglckf/ejournal/upfiles/08-27/27k-5239(ps).pdf]