Efficacy of heterogenic deproteinized cancellous bone combined with bone morphogenetic protein and fibrin in repairing rabbit radial defects

Zhang Xu-yuan1, Liu Xing-yan2, Ge Bao-feng2, Chen Ke-ming2, Deng Gang1, Bai Meng-hai2

1Surgery Department, Clinical Medical College of Lanzhou University, Lanzhou   730000, Gansu Province, China; 2Department of Orthopedics, General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou   730050, Gansu Province, China

Zhang Xu-yuan★, Studying for master’s degree, Attending physician, Surgery Department, Clinical Medical College of Lanzhou University, Lanzhou   730000, Gansu Province, China
zxy13919098623@ 126.com

Correspondence to: Liu Xing-yan, Chief physician, Professor, Department of Orthopedics, General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou   730050, Gansu Province, China
redcrafter@126.com

Supported by: Military Medical Research Fund during the Eleventh Five-year Plan, No. 06G033*

Received: 2008-03-29
Accepted: 2008-05-24

Abstract
BACKGROUND: Heterogenic deproteinized cancellous bones possess good biomechanical property and provide temporal mechanical supports for the weight-bearing tissues.
OBJECTIVE: To study the effect of the composite combining heterogenic deproteinized cancellous bone with bone morphogenetic protein (BMP) and fibrin to repair segmental bone defects.
DESIGN, TIME AND SETTING: The animal experiment of randomization design was performed at War Wounds and Bone Disease Key Laboratory of Chinese PLA at High Altitude from October 2006 to October 2007.
MATERIALS: Bone and periosteum defects were created in the bilateral radius of 45 New Zealand rabbits. Heterogenic deproteinized cancellous bones, obtained from fresh vertebrae of sheep, were prepared into cancellous bone particles which were 0.5 cm × 0.5 cm × 0.5 cm in size and processed into cleaning, defatting, part decalcification and deproteinization. BMP was extract from fresh cortical bone of calf. Fibrin was extract from the blood of allogeneic rabbits.
METHODS: Forty-five rabbits were randomly divided into three groups (n=15): composite group implanted with the complex of heterogenic deproteinized cancellous bone/BMP/fibrin, heterogenic deproteinized cancellous bone group implanted with heterogenic deproteinized cancellous bone, and control group implanted with nothing.
MAIN OUTCOME MEASURES: The healing of bone defects was detected through gross observation, radiology and histology at 2, 4, 8, 12, 16 weeks after operation. New bone quantitative analysis was conducted according to the ratio of new bone formation area to the total area of vision.
RESULTS: At 16 weeks after operation, the defects were perfectly healed in the composite group, which was better than heterogenic deproteinized cancellous bone and control group with regard to the levels of bone formation, quantity of bone regeneration and reconstruction of marrow cavity. The bone union emerged in the heterogenic deproteinized cancellous bone group. However, its marrow cavity was not recanalized. No osseous tissue healing was observed in the control group.
CONCLUSION: The composites of heterogenic deproteinized cancellous bone/BMP/fibrin can be effective to repair segmental bone defects.

Zhang XY, Liu XY, Ge BF, Chen KM, Deng G, Bai MH.Efficacy of heterogenic deproteinized cancellous bone combined with bone morphogenetic protein and fibrin in repairing rabbit radial defects.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(27):5214-5218 [www.zglckf.com/zglckf/ejournal/upfiles/08-27/27k-5214(ps).pdf]