【Title】

“Human umbilical cord mesenchymal stem cells transplantation improves lower limb ischemia of diabetic rabbits ”living pictures

【Source】

Journal of Clinical Rehabilitative Tissue Engineering Research  January 1, 2011  Vol.15 No. 1，33-36

【Author】

Fan Ming-shi, Yang Xiao-feng, Wu Yan-xiang, Zhang Yi-bin, Xu Yi-feng, Song Nan, Huang Sheng-nan, Zhang Yue

【Setting】

Department of Hematology and Endocrinology, the 463 Hospital of Chinese PLA, Shenyang  110042, Liaoning Province, China

【Found】

【Abstract】

BACKGROUND: The transplantation of bone marrow and autologous peripheral blood stem cells into the muscles of lower limbs of diabetes patients with lower limb ischemia can promote blood vessel regeneration, improve and recover the blood flow of affected limbs of diabetes patients with lower limb ischemia. However, the collection has great risk, and requires high levels in patients’ age, body condition and psychological reception degree. Compared with bone marrow and autologous peripheral blood stem cells, umbilical cord resource is abundant; cell collection is simple; immunogenicity is weak.

OBJECTIVE: To investigate the feasibility that transplantation of human umbilical cord mesenchymal stem cells (HUCMSCs) for treatment of diabetic rabbit of lower limbs extremity arterial. 

METHODS: Models of diabetic rabbits were established. Femoral arteries of lower limbs were ligated. Prepared HUCMSCs in sign of DIL were directly injected into the left hindlimb four-headed thigh muscles in diabetic rabbit models (cell quantity was 1.67×10⁶ per point). Saline was directly injected into right lower limbs. At 2 and 4 weeks, adductor and gastrocnemius nuscle of the bilateral hindlimbs were collected. Angiogenesis of pathological sections was observed. Capillary density and skin temperature were measured using immunohistochemical staining for CD31.

RESULTS AND CONCLUSION: Pathological sections exhibited that collateral vessels were significantly increased in the transplantation group compared with control group. Immunohistochemical staining for CD31 demonstrated that the capillary density was significantly higher in the transplantation group compared with control group. The skin temperature was significantly greater in the transplantation group compared with control group (P ≤ 0.05). Results suggested that HUCMSCs transplantation in the treatment of diabetic rabbit of lower limbs ischemia is an effective measure.

【JPG pictures】

8 figures click

Figure 1  The first generation of human umbilical cord mesenchymal stem cells presented irregular shape such as polygon (×100)

Figure 2  The third generation of human umbilical cord mesenchymal stem cells grow uniformity under lens, appears long arris and whirlpool shape (×100)

Figure 3  No immunoreactive stem cell by DIL dye in the control group (Immunofluorescence staining, ×200)

Figure 4  A great deal of immunoreactive stem cell by DIL dye in the transplantation group among muscle (Immunofluorescence staining, ×200)

Figure 5  Few capillary vessels in the control group (Hematoxylin-eosin staining, ×200)

Figure 6  A lot of capillary vessel in the transplantation group, distinctness compared with the control group (Hematoxylin-eosin staining, ×200)

Figure 7  Few capillary vessel in the control group (Immunohistochemical staining, ×200)

Figure 8  A lot of capillary vessel in the transplantation group, distinctness compared with the control group (Immunohistochemical staining, ×200)

【Full text】